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Dinitrophenol Coupled Albumin anticorps

L’anticorps Lapin Polyclonal anti- a été validé pour ID et IEP. Il convient pour détecter dans des échantillons de Singe.
N° du produit ABIN458297

Aperçu rapide pour Dinitrophenol Coupled Albumin anticorps (ABIN458297)

Antigène

Dinitrophenol Coupled Albumin

Reactivité

Singe

Hôte

Lapin

Clonalité

Polyclonal

Application

Immunodiffusion (ID), Immunoelectrophoresis (IEP)
  • Specificité

    Inter-species cross reactivity is a normal feature of antibodies to serum proteins, since homologous proteins of different species frequently share antigenic determinants. of this antiserum has not been tested in detail, however a reaction has been observed with human albumin, chicken ovalbuminDNP, but not with native chicken ovalbumin.

    Attributs du produit

    Precipitating polyclonal rabbit antiserum to monkey dinitrophenol coupled albumin

    Purification

    Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required, to eliminate antibodies reacting with other serum proteins. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum.

    Immunogène

    Purified albumin isolated from pooled monkey serum, coupled to dinitrophenol. Freund’s complete adjuvant is used in the first step of the immunization procedure.

    Isotype

    IgG
  • Indications d'application

    In precipitating techniques as immunoelectrophoresis and radial immunodiffusion (Ouchterlony and Mancini) and electroimmunodiffusion (Laurell) to identify the presence of albumin or albuminDNP in monkey serum or other body fluids and to determine its concentration. This antiserum is not intended for use in non-precipitating antibody-binding or other highly sensitive assays, but this does not exclude such use if proper controls are included.

    Restrictions

    For Research Use only
  • Format

    Lyophilized

    Concentration

    Total protein and IgG concentrations in the antiserum are comparable to those of pooled normal rabbit serum. No foreign proteins added. Antibody titre: Precipitin titre 1:32 when tested against pooled normal monkey serum in agar-block immunodiffusion titr

    Buffer

    Delipidated, heat inactivated, lyophilized, stable whole antiserum

    Agent conservateur

    Without preservative

    Stock

    4 °C/-20 °C

    Stockage commentaire

    The lyophilized antiserum is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Reconstitute the lyophilized antiserum by add ing 1 ml sterile distilled water. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at +4°C, not ref rozen, and preferably used the same day.
  • Antigène

    Dinitrophenol Coupled Albumin

    Sujet

    The reactivity of the antiserum is restricted to albumin and albuminDNP as tested in immunoelectrophoresis and radial immunodiffusion. A characteristic single precipitin line is obtained with normal serum which shows a reaction of identity with the precipitin line with purified albumin
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